In vitro Characterization of a Trichoplusia ni Single Nucleocapsid Nuclear Polyhedrosis Virus
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چکیده
منابع مشابه
Characterization of DNA Polymerase Activity in Trichoplusia ni Cells Infected with Autographa californica Nuclear Polyhedrosis Virus
Nuclei isolated from Trichoplusia ni cells (TN-368) infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) were used to study the replication of viral DNA. Based on DNA : DNA hybridization data, virus-specific DNA polymerase activity was insensitive to aphidicolin and novobiocin and was inhibited by ddTTP and N-ethylmaleimide. The data indicate that the AcMNPV-specific DNA pol...
متن کاملNucleotide sequence of the polyhedrin gene region of Helicoverpa zea single nucleocapsid nuclear polyhedrosis virus: placement of the virus in lepidopteran nuclear polyhedrosis virus group II.
The polyhedrin gene (polh) of Helicoverpa zea single nucleocapsid nuclear polyhedrosis virus (HzSNPV) was identified and shown by sequence analysis of the EcoRI I genomic fragment to encode a 246 amino acid polypeptide that has greater than 80% sequence identity to known polyhedrins. It is preceded by an AT-rich region containing the conserved late promoter motif TAAG, which was identified as a...
متن کاملBaculovirus Replication: Stimulation of Thymidine Kinase and DNA Polymerase Activities in Spodopterafrugiperda Cells Infected with Trichoplusia ni Nuclear Polyhedrosis Virus
Baculoviruses are large DNA-containing viruses which replicate in the nucleus of invertebrate cells. The viruses contain supercoiled DNA of tool. wt. about 8 x l 0 7 (Bud & Kelly, 1977) and the multiply enveloped nuclear polyhedrosis virus of Trichoplusia ni used in this study has a genome size of 8.3 x 107 (Bud & Kelly, 1980). Little is known about the replication of baculoviruses in molecular...
متن کاملLocation and nucleotide sequence of the Orgyia pseudotsugata single nucleocapsid nuclear polyhedrosis virus polyhedrin gene.
A restriction endonuclease map was determined for the Orgyia pseudotsugata single nucleocapsid nuclear polyhedrosis virus (SNPV) genome. The order of the fragments generated by the enzymes BglII, BamHI and XbaI was analysed using double digestion of the total genome and digestion of isolated restriction fragments. The location of the polyhedrin gene was then determined using a cloned polyhedrin...
متن کاملCharacterization of nuclear polyhedrosis virus DNAs.
The nuclear polyhedrosis virus DNAs characterized and compared in this study consist of the singly-enveloped nucleocapsids (SNPV) of Trichoplusia ni and the bundles of nucleocapsids common to a single envelope (MNPV) from Spodoptera frugiperda and Rachiplusia ou. The SNPV and MNPV DNAs are very similar in hydrodynamic properties and molecular weights. In addition, the NPV DNAs are similar in si...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1996
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-77-9-2303